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doi: 10.3390/ijms20184354

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[61] for the early diagnosis of breast cancer

First identified in human plasma, its concentration declines significantly with agefrom approximately 200 ng/mL in youth to 80 ng/mL by the age of 60 (Pickart and Margolina 2018)

This is true whether you opened it yesterday or three months ago: what matters is the date of the first puncture, not how much liquid is left