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Fluorescence microscopy images were obtained with an EVOS FL Auto Imaging System (Thermo Fisher Scientific, USA), and fluorescence image intensity was analyzed with ImageJ software

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More peptide stays free, and the half-life stretches from roughly 12-15 hours for native IGF-1 to about 20-30 hours for IGF-1 LR3

2.4.5.2 Liquid chromatography-mass spectrometry analysis conditions The analyzing instrument was a liquid-mass spectrometer system consisting of ACQUITY UPLC I-Class plus ultra-high performance liquid tandem high-resolution mass spectrometer

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