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Real-time PCR was conducted for amplification with specific primers for the chondrogenic markers COL2A1 (Forward: 5-TTCAGCTATGGAGATGACAATC-3 and Reverse: 5-AGAGTCCTAGAGTGACTGAG-3), ACAN (Forward: 5-AGCCTGCGCTCCAATGACT-3 and Reverse: 5-TAATGGAACACGATGCCTTTCA-3), and SOX9 (Forward: 5-TTCCGCGACGTGGACAT-3 and Reverse: 5-TCAAACTCGTTGACATCGAAGGT-3)

Next the lateral epicondyle is identified and solution is peppered here